Analysis of relative gene expression data using real-time.
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I guess what I'm wanting to do is a sort of ddCt Analysis but as my primers are 100% efficient I'm not allowed to. DART is almost there, it takes each reaction and calculates the efficiency for each well, but then just averages all the expression and efficiencies.
Should I use the deltaCT value instead? Or DDCT even? The results differ in the statistical analysis. Should I use the delta CT SD perhaps, instead of SE? I'm trying to parse statistical papers and explanations but I'm a bit stupid, and english isn't my first language. Thank you for any input! I've tried to keep it short but I can elaborate if.
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